 | Related Articles |
Use of influenza A viruses expressing reporter genes to assess the frequency of double infections in vitro.
J Gen Virol. 2012 Aug;93(Pt 8):1645-8
Authors: Bodewes R, Nieuwkoop NJ, Verburgh RJ, Fouchier RA, Osterhaus AD, Rimmelzwaan GF
Abstract
Exchange of gene segments between mammalian and avian influenza A viruses may lead to the emergence of potential pandemic influenza viruses. Since co-infection of single cells with two viruses is a prerequisite for reassortment to take place, we assessed frequencies of double-infection in vitro using influenza A/H5N1 and A/H1N1 viruses expressing the reporter genes eGFP or mCherry. Double-infected A549 and Madin-Darby canine kidney cells were detected by confocal microscopy and flow cytometry.
PMID: 22535774 [PubMed - indexed for MEDLINE]
